Huang Group
黄岩谊实验室

Tianyi Chang, Wuji Han, Mengcheng Jiang, Jizhou Li, Zhizhao Liao, Mingchuan Tang, Jianyun Zhang, Jie Shen, Zitian Chen, Peng Fei, Xianwen Ren, Yuhong Pang, Guanbo Wang, Jianbin Wang,* Yanyi Huang.*.

Rapid and Signal Crowdedness-Robust In-Situ Sequencing through Hybrid Block Coding.

Proceedings of the National Academy of Sciences of the United States of America 120 (47), e2309227120 (2023) . doi: 10.1073/pnas.2309227120

Xinyang Shao, Yanyi Huang,* Guanbo Wang*.

Microfluidic devices for protein analysis using intact and top-down mass spectrometry.

VIEW 4 20220032 (2023) . doi: 10.1002/VIW.20220032

Shanglin Li, Bao Li, Xinyue Li, Ce Liu, Xiao Qi, Yin Gu, Baobao Lin, Lingli Sun, Lan Chen, Bingqian Han, Jiazhen Guo, Yanyi Huang, Shuangsheng Wu, Lili Ren, Jianbin Wang, Jingwei Bai, Jianxin Ma, Maosheng Yao, Peng Liu.

An ultrasensitive and rapid “sample-to-answer” microsystem for on-site monitoring of SARS-CoV-2 in aerosols using “in situ” tetra-primer recombinase polymerase amplification.

Biosensors and Bioelectronics 219 114816 (2023) . doi: 10.1016/j.bios.2022.114816

Xiannian Zhang,* Zhenwei Qian, Mengcheng Jiang, Wentao Li, Yanyi Huang,* Yongfan Men*.

Design and High-Resolution Analysis of an Efficient Periodic Split-and-Recombination Microfluidic Mixer.

Micromachines 13 1720 (2022) . doi: 10.3390/mi13101720

Wen Li, Lingxiao Chaihu, Jialu Jiang, Bizhu Wu, Xuan Zheng, Rongrong Dai, Ye Tian, Yanyi Huang, Guanbo Wang*, Yongfan Men*.

Microfluidic Platform for Time-Resolved Characterization of Protein Higher-Order Structures and Dynamics Using Top-Down Mass Spectrometry.

Analytical Chemistry 94 (21), 7520-7527 (2022) . doi: 10.1021/acs.analchem.2c00077

Mengcheng Jiang, Peiyu Liao, Yue Sun, Xinyang Shao, Zitian Chen, Peng Fei, Jianbin Wang, Yanyi Huang*.

Rotational scan digital LAMP for accurate quantitation of nucleic acids.

Lab on a Chip 21 (11), 2265-2271 (2021) . doi: 10.1039/D1LC00114K

Yongxi Zhao, Xiaolei Zuo, Qian Li, Feng Chen, Yan-Ru Chen, Jinqi Deng, Da Han, Changlong Hao, Fujian Huang, Yanyi Huang, Guoliang Ke, Hua Kuang, Fan Li, Jiang Li, Min Li, NaLi, Zhenyu Lin, Dingbin Liu, Juewen Liu, Libing Liu, Xiaoguo Liu, Chunhua Lu, Fang Luo, Xiuhai Mao, Jiashu Sun, Bo Tang, Fei Wang, Jianbin Wang, Lihua Wang, Shu Wang, Lingling Wu, Zai-Sheng Wu, Fan Xia, C Xu, Y Yang, B-F Yuan, Q Yuan, C Zhang, Z Zhu, Chaoyong Yang, Xiao-Bing Zhang, Huanghao Yang, Weihong Tan, Chunhai Fan.

Nucleic Acids Analysis.

Science China Chemistry 64 (2), 171-203 (2021) . doi: 10.1007/s11426-020-9864-7

Peiyu Liao,# Mengcheng Jiang,# Zitian Chen, Fangli Zhang, Yue Sun, Jun Nie, Meijie Du, Jianbin Wang,* Peng Fei,* Yanyi Huang*.

3D digital PCR through light-sheet imaging of optically cleared emulsion.

Proceedings of the National Academy of Sciences of the United States of America 117 (41), 25628-25633 (2020) . doi: 10.1101/739243

廖珮宇，黄岩谊*.

分而治之——微液滴中的核酸分析化学研究（综述）.

中国科学 化学 50 (10), 1439-1448 (2020) . doi: 10.1360/SSC-2020-0133

Fangli Zhang, Peiyu Liao, Yue Sun, Zitian Chen, Yuhong Pang, Yanyi Huang*.

Surfactant and Oil Formulations for Monodisperse Droplet Emulsion PCR.

Lab on a Chip 20 (13), 2328-2333 (2020) . doi: 10.1039/D0LC00052C

Chee Meng Benjamin Ho,# Qi Sun,# Adrian J. T. Teo, David Wibowo, Yongsheng Gao, Jun Zhou, Yanyi Huang, Say Hwa Tan,* Chun-Xia Zhao*.

Development of a Microfluidic Droplet-Based Microbioreactor for Microbial Cultivation.

ACS Biomaterials Science & Engineering 6 (6), 3630-3637 (2020) . doi: 10.1021/acsbiomaterials.0c00292

Kui Han,# Long Mei,# Ruoyu Zhong, Yuhong Pang, Eric Erquan Zhang,* Yanyi Huang*.

A Microfluidic Approach for Experimentally Modelling the Intercellular Coupling System of a Mammalian Circadian Clock at Single-cell Level.

Lab on a Chip 20 (7), 1204-1211 (2020) . doi: 10.1039/D0LC00140F

( featured as inside front cover. )

Juanli Yun, Xiaowei Zheng, Peng Xu, Xu Zheng, Jingyue Xu, Chen Cao, Yusi Fu, Bingxue Xu, Xin Dai, Yi Wang, Hongtao Liu, Qiaolian Yi, Yaxin Zhu, Jian Wang, Li Wang, Zhiyang Dong, Li Huang,* Yanyi Huang,* Wenbin Du*.

Interfacial Nanoinjection-Based Nanoliter Single-Cell Analysis.

Small 16 (9), 1903739 (2020) . doi: 10.1002/smll.201903739

Yusi Fu,# Fangli Zhang,# Xiannian Zhang, Junlong Yin, Meijie Du, Mengcheng Jiang, Lu Liu, Jie Li, Yanyi Huang,* Jianbin Wang*.

High-throughput single-cell whole-genome amplification through centrifugal emulsification and eMDA.

Communications Biology 2 147 (2019) . doi: 10.1038/s42003-019-0401-y

Xiannian Zhang,# Tianqi Li,# Feng Liu,# Yaqi Chen,# Jiacheng Yao, Zeyao Li, Yanyi Huang,* Jianbin Wang*.

Comparative analysis of droplet-based ultra-high-throughput single-cell RNA-seq systems.

Molecular Cell 73 (1), 130-142 (2019) . doi: 10.1016/j.molcel.2018.10.020

Shuang Geng and Yanyi Huang*.

From mouth pipetting to microfluidics: the evolution of technologies for picking healthy single cells.

Advanced Biosystems 2 (9), 1800099 (2018) . doi: 10.1002/adbi.201800099

Zitian Chen, Wenxiong Zhou, Shuo Qiao, Li Kang, Haifeng Duan, X. Sunney Xie, Yanyi Huang*.

Highly accurate fluorogenic DNA sequencing with information theory-based error correction.

Nature Biotechnology 35 (12), 1170-1178 (2017) . doi: 10.1038/nbt.3982

Peiyu Liao and Yanyi Huang*.

Digital PCR: Endless Frontier of ‘Divide and Conquer’ (Perspective).

Micromachines 8 231 (2017) . doi: 10.3390/mi8080231

( featured as front cover )

Angela R. Wu,* Jianbin Wang,* Aaron M. Streets,* Yanyi Huang*.

Single-Cell Transcriptional Analysis (Review).

Annual Review of Analytical Chemistry 10 439-462 (2017) . doi: 10.1146/annurev-anchem-061516-045228

Chunhong Zheng,# Xiannian Zhang,# Chunmei Li, Yuhong Pang,* Yanyi Huang*.

A microfluidic device for studying controllable hydrodynamic flow induced cellular responses.

Analytical Chemistry 89 (6), 3710-3715 (2017) . doi: 10.1021/acs.analchem.7b00013

Hengdong Xi, Hao Zheng, Wei Guo, Alfonso M. Ganan-Calvo, Ye Ai, Chia-Wen Tsao, Jun Zhou, Weihua Li, Yanyi Huang, Nam-Trung Nguyen, and Say Hwa Tan*.

Active droplet sorting in microfluidics: a review.

Lab on a Chip 17 (5), 751-771 (2017) . doi: 10.1039/C6LC01435F

( featured as back cover of the issue )

Zitian Chen, Peiyu Liao, Fangli Zhang, Mengcheng Jiang, Yusen Zhu, Yanyi Huang*.

Centrifugal Micro-Channel Array Droplet Generation for Highly Parallel Digital PCR.

Lab on a Chip 17 (2), 235-240 (2017) . doi: 10.1039/C6LC01305H

( featured as inside front cover )

Yusi Fu, He Chen, Lu Liu, Yanyi Huang*.

Single cell total RNA sequencing through isothermal amplification in picoliter-droplet emulsion.

Analytical Chemistry 88 (22), 10795-10799 (2016) . doi: 10.1021/acs.analchem.6b02581

Zitian Chen, Yusi Fu, Fangli Zhang, Lu Liu, Naiqing Zhang, Dong Zhou, Junrui Yang, Yuhong Pang, Yanyi Huang*.

Spinning micro-pipette liquid emulsion generator for single cell whole genome amplification.

Lab on a Chip 16 (23), 4512-4516 (2016) . doi: 10.1039/C6LC01084A

Charles M. Schroeder,* Sarah Köster, Yanyi Huang.

Emerging Investigators 2016: discovery science meets technology (Editorial).

Lab on a Chip 16 (16), 2974-2976 (2016) . doi: 10.1039/C6LC90076C

Chen Cao, Dong Zhou, Tao Chen, Aaron M. Streets,* Yanyi Huang*.

Label-free Digital Quantification of Lipid Droplets in Single Cells by Stimulated Raman Microscopy on a Microfluidic Platform.

Analytical Chemistry 88 (9), 4931-4939 (2016) . doi: 10.1021/acs.analchem.6b00862

虞之龙，黄岩谊*.

集成微流控芯片在单细胞测序样品制备中的应用（综述）.

中国科学 化学 45 (11), 1090-1101 (2015) . doi: 10.1360/N032015-00100

( 封面论文 )

Yusi Fu, Chunmei Li, Sijia Lu, Wenxiong Zhou, Fuchou Tang, X. Sunney Xie,* Yanyi Huang*.

Uniform and accurate single-cell sequencing based on emulsion whole-genome amplification.

Proceedings of the National Academy of Sciences of the United States of America 112 (38), 11923-11928 (2015) . doi: 10.1073/pnas.1513988112

Jie Shen, Changzu Cai, Zhilong Yu, Yuhong Pang, Ying Zhou, Lili Qian, Wensheng Wei,* Yanyi Huang*.

A microfluidic live cell assay to study anthrax toxin induced cell lethality assisted by conditioned medium.

Scientific Reports 5 8651 (2015) . doi: 10.1038/srep08651

Xiannian Zhang, Zitian Chen, Yanyi Huang*.

A valve-less microfluidic peristaltic pumping method.

Biomicrofluidics 9 (1), 014118 (2015) . doi: 10.1063/1.4907982

Jie Shen,# Dongqing Jiang,# Yusi Fu,# Xinglong Wu,# Hongshan Guo, Binxiao Feng, Yuhong Pang, Aaron M. Streets, Fuchou Tang,* Yanyi Huang*.

H3K4me3 epigenomic landscape derived from ChIP-Seq of 1,000 mouse early embryonic cells.

Cell Research 25 (1), 143-147 (2015) . doi: 10.1038/cr.2014.119

Aaron M. Streets and Yanyi Huang*.

How deep is enough in single-cell RNA-seq? (Commentary).

Nature Biotechnology 32 (10), 1005-1006 (2014) . doi: 10.1038/nbt.3039

Zhilong Yu, Sijia Lu, Yanyi Huang*.

Microfluidic Whole Genome Amplification Device for Single Cell Sequencing.

Analytical Chemistry 86 (19), 9386-9390 (2014) . doi: 10.1021/ac5032176

Dino Di Carlo,* Yanyi Huang, Helene Andersson-Svahn.

Emerging investigators: new challenges spawn new innovations (Editorial).

Lab on a Chip 14 (15), 2599-2600 (2014) . doi: 10.1039/c4lc90058h

Aaron M. Streets,# Xiannian Zhang,# Chen Cao, Yuhong Pang, Xinglong Wu, Liang Xiong, Lu Yang, Yusi Fu, Liang Zhao,* Fuchou Tang,* Yanyi Huang*.

Microfluidic single-cell whole transcriptome sequencing.

Proceedings of the National Academy of Sciences of the United States of America 111 (19), 7048-7053 (2014) . doi: 10.1073/pnas.1402030111

Chunhong Zheng, Hongwei Zhou, Xinxing Liu, Yuhong Pang, Bo Zhang, Yanyi Huang*.

Fish in chips: an automated microfluidic device to study drug dynamics in vivo using zebrafish embryos.

Chemical Communications 50 (8), 981-984 (2014) . doi: 10.1039/C3CC47285J

Aaron M. Streets and Yanyi Huang*.

Microfluidics for biological measurements with single-molecule resolution (Review Article).

Current Opinion in Biotechnology 25 (1), 69-77 (2014) . doi: 10.1016/j.copbio.2013.08.013

Bo Xian,# Jie Shen,# Weiyang Chen,# Na Sun, Nan Qiao, Dongqing Jiang, Tao Yu, Yongfan Men, Zhijun Han, Yuhong Pang, Matt Kaeberlein, Yanyi Huang,* Jing-Dong J. Han*.

WormFarm: A quantitative control and measurement device towards automated C. elegans aging analysis.

Aging Cell 12 (3), 398-409 (2013) . doi: 10.1111/acel.12063

Aaron M. Streets and Yanyi Huang*.

Chip in a Lab: Microfluidics for Next Generation Life Science Research (Perspective Article).

Biomicrofluidics 7 (1), 011302 (2013) . doi: 10.1063/1.4789751

Peng Fei,# Zitian Chen,# Yongfan Men, Ang Li, Yiran Shen, Yanyi Huang*.

A Compact Optofluidic Cytometer with Integrated Liquid-core/PDMS-cladding Waveguides.

Lab on a Chip 12 (19), 3700-3706 (2012) . doi: 10.1039/C2LC40329C

Chunhong Zheng,# Zhilong Yu,# Ying Zhou,# Louis Tao, Yuhong Pang, Tao Chen, Xiannian Zhang, Haiwei Qiu, Hongwei Zhou, Zitian Chen, Yanyi Huang*.

Live cell imaging analysis of the epigenetic regulation of the human endothelial cell migration at single cell resolution.

Lab on a Chip 12 (17), 3063-3072 (2012) . doi: 10.1039/C2LC40192D

( featured by Lab on a Chip blog as a "HOT article" )

Chunhong Zheng, Jingwen Wang, Yuhong Pang, Jianbin Wang, Wenbin Li, Zigang Ge,* Yanyi Huang*.

High-throughput immunoassay through in-channel microfluidic patterning.

Lab on a Chip 12 (14), 2487-2490 (2012) . doi: 10.1039/c2lc40145b

( featured as inside front cover )

Yongfan Men, Yusi Fu, Zitian Chen, Peter A. Sims, William J. Greenleaf, Yanyi Huang*.

Digital polymerase chain reaction in an array of femtoliter PDMS microreactors.

Analytical Chemistry 84 (10), 4262-4266 (2012) . doi: 10.1021/ac300761n

Wentao Li, Tao Chen, Zitian Chen, Peng Fei, Zhilong Yu, Yuhong Pang, Yanyi Huang*.

Squeeze-chip: A finger controlled microfluidic flow network device and its application to biochemical assays.

Lab on a Chip 12 (9), 1587-1590 (2012) . doi: 10.1039/C2LC40125H

( featured as inside front cover )

Chunhong Zheng, Gui’e Chen, Yuhong Pang, Yanyi Huang*.

An Integrated microfluidic device for long-term culture of isolated single mammalian cells.

Science China Chemistry 55 (4), 502–507 (2012) . doi: 10.1007/s11426-012-4493-1

( featured as front cover )

Ying Zhou, Yuhong Pang, Yanyi Huang*.

Openly Accessible Microfluidic Liquid Handlers for Automated High-throughput Nanoliter Cell Culture.

Analytical Chemistry 84 (5), 2576-2584 (2012) . doi: 10.1021/ac203469v

Chunhong Zheng,# Liang Zhao,# Gui'e Chen, Ying Zhou, Yuhong Pang, Yanyi Huang*.

Quantitative Study of the Dynamic Tumor-Endothelial Cell Interactions through an Integrated Microfluidic Coculture System.

Analytical Chemistry 84 (4), 2088-2093 (2012) . doi: 10.1021/ac2032029

Jie Shen, Ying Zhou, Tu Lu, Junya Peng, Zhixiang Lin, Lei Huang, Yuhong Pang, Li Yu, Yanyi Huang*.

An Integrated Chip for Immunofluorescence and Its Application to Analyze Lysosomal Storage Disorders.

Lab on a Chip 12 (2), 317-324 (2012) . doi: 10.1039/C1LC20845D

Zeyong Zhi, Pengcheng Liu, Peng Wang, Yanyi Huang, Xin Sheng Zhao*.

Domain Specific Folding Kinetics of Staphylococcal Nuclease Observed through Single-Molecule FRET in Microfluidic Mixer.

ChemPhysChem 12 (18), 3515-3518 (2011) . doi: 10.1002/cphc.201100652

Hanshuo Zhang,# Yang Hao,# Junyu Yang,# Ying Zhou, Juan Li, Shenyi Yin, Changhong Sun, Ming Ma, Yanyi Huang, Jianzhong Jeff Xi*.

Genome-wide Functional Screening of miR-23b as a Pleiotropic Modulator Suppressing Cancer Metastasis.

Nature Communications 2 554 (2011) . doi: 10.1038/ncomms1555

Peng Fei, Zi He, Chunhong Zheng, Tao Chen, Yongfan Men, Yanyi Huang*.

Discretely Tunable Optofluidic Compound Microlenses.

Lab on a Chip 11 (17), 2835-2841 (2011) . doi: 10.1039/c1lc20425d

( featured as back cover, highlighted in Nature Photonics )

赵亮，申洁，周宏伟，黄岩谊*.

集成微流控芯片（综述）.

科学通报 56 (23), 1855-1870 (2011) . doi: 10.1360/972010-1955

Ze-Yong Zhi, Peng-Cheng Liu, Yan-Yi Huang, Xin-Sheng Zhao*.

A Microfluidic Mixer for Single-Molecule Kinetics Experiments.

Acta Physico-Chimica Sinica 27 (8), 1990-1995 (2011) .

Zuoyan Han, Wentao Li, Yanyi Huang, Bo Zheng*.

Measuring rapid enzymatic kinetics by electrochemical method in droplet-based microfluidic devices with pneumatic valves.

Analytical Chemistry 81 (14), 5840-5845 (2009) . doi: 10.1021/ac900811y

Jianbin Wang,# Ying Zhou,# Haiwei Qiu, Huang Huang, Changhong Sun, Jianzhong Xi,* Yanyi Huang*.

A chip-to-chip nanoliter microfluidic dispenser.

Lab on a Chip 9 (13), 1831-1835 (2009) . doi: 10.1039/b901635j

( featured as back cover )

Chunhu Xu, Wei Sun, Chao Zhang, Yanchun Bai ,Chenjie Fang, Wentao Li, Yanyi Huang, Chunhua Yan*.

Chemical approaches for mimicking logic functions within fluorescent MPT dyes.

Science in China Series B: Chemistry 52 (6), 700-714 (2009) . doi: 10.1007/s11426-009-0097-9

Yuanzi Wu, Yanyi Huang, and Hongwei Ma*.

A Facile Method for Permanent and Functional Surface Modification of Poly(dimethylsiloxane).

Journal of the American Chemical Society 129 (23), 7226-7227 (2007) . doi: 10.1021/ja071384x

Yanyi Huang,# Piero Castrataro,# Cheng-Chung Lee,# Stephen R. Quake*.

Solvent resistant microfluidic DNA synthesizer.

Lab on a Chip 7 (1), 24-26 (2007) .

Lin Zhu,* Yanyi Huang, Amnon Yariv.

Integrated microfluidic variable optical attenuator.

Optics Express 13 (24), 9916-9921 (2005) .